Comparability of a Three-Dimensional Structure in Biopharmaceuticals Using Spectroscopic Methods
نویسندگان
چکیده
منابع مشابه
Comparability of a Three-Dimensional Structure in Biopharmaceuticals Using Spectroscopic Methods
Protein structure depends on weak interactions and covalent bonds, like disulfide bridges, established according to the environmental conditions. Here, we present the validation of two spectroscopic methodologies for the measurement of free and unoxidized thiols, as an attribute of structural integrity, using 5,5'-dithionitrobenzoic acid (DTNB) and DyLight Maleimide (DLM) as derivatizing agents...
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ژورنال
عنوان ژورنال: Journal of Analytical Methods in Chemistry
سال: 2014
ISSN: 2090-8865,2090-8873
DOI: 10.1155/2014/950598